Journal: eLife

Article Title: An unexpected role of neutrophils in clearing apoptotic hepatocytes in vivo

doi: 10.7554/eLife.86591

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , IL-6Rα shRNA plasmid , SCBT , sc-35663-SH , .

Techniques: Recombinant, shRNA, Plasmid Preparation, In Situ, Enzyme-linked Immunosorbent Assay, Software, Imaging, Light Microscopy

Journal: The Journal of Biological Chemistry

Article Title: Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans -signaling

doi: 10.1074/jbc.M116.762021

Figure Lengend Snippet: EBI3-induced B9 cell proliferation can be inhibited by anti-IL-6, anti-gp130, and 25F10, a mAb that block IL-6 trans-signaling. Untransfected B9 cells were incubated with mEBI3 (500 ng/ml), IL-6 (0.01 ng/ml), or IL-27 (100 ng/ml) with or without anti-gp130 (A), anti-IL-6 (B), 25F10 (C), or anti-IL-6R (D) mAbs. Proliferation was measured as described in the legend to Fig. 2. Error bars indicate the S.D. of triplicate cultures. Statistical differences between IL-6 ± mAbs and EBI3 ± mAbs were determined using Student's t test. ***, p < 0.001; **, p < 0.01. The data are representative of three independent experiments.

Article Snippet: For inhibitory studies, IL-6 or gp130 targeting antibodies (5 μg/ml) or the anti-IL6·IL-6Rα complex 25F10 mAb (10 μg/ml; NovImmune, Plan-Les-Ouates, Switzerland) were added as indicated.

Techniques: Blocking Assay, Incubation